H
Hariseldon
Although your pump is turning, it may not be working as well as it should, perhaps the impellar is damaged or broken. It's possible that what you have is hot water convecting to the top floor and you aren't getting full circulation
You may well be right but I think it is unlikely, because removing a rad a month ago caused the same problem and shifting an air lock then, not only got everything working but got the rads on the lowest floor to work properly for the first time.
Removing a lower floor rad brought this problem on and it would be a bit of coincidence if the pump had suffered a problem at the same moment. I will have a go at letting some water out of the affected rads tomorrow and see what happens.